Simultaneous estimation of epigallocatechin, fisetin, and quercetin in Acacia suma and its potential against postprandial hyperglycemia

Anti-diabetic activity of the plants may follow one or other mechanism such as their ability to restore pancreatic tissue function increase in insulin output inhibiting intestinal absorption glucose enzymes like alpha-amylase, alpha-glucosidase, etc. The alpha-amylase and alpha-glucosidase inhibition assays were performed analyze anti-diabetic potential Acacia suma. RP-HPLC method was developed validated for simultaneous estimation Epigallocatechin (EGC), Fisetin (FT), Quercetin (QT) hydroalcoholic extract (HAE) suma formulations. using Luna C18 column with 20µl sample injection volume. Whereas, acetonitrile phosphate buffer (0.1%, 30:70 v/v, pH 2.7) used resolution mobile phase temperature 40 OC flow rate 1 ml/min. IC50 found be 12.5µg/ml 93.79µg/ml respectively compared standards. simple, specific, precise linear regression coefficient (R2 = 0.999) over selected range concentration (2.5-160 μg/mL) having detection limits 0.076, 0.012, 0.010 μg/mL quantification 0.231, 0.038, 0.031 EGC, FT QT respectively. In-vitro assay support traditional claim plant treatment metabolic disorders. established demonstrates easy determination FT, is effectively studied by a gradient elution system.